AIM: To study the restorative effect of Coriolus versicolor polysaccharides (CVP) on spleen injury induced by gamma-ray irradiation in mice. METHODS: ICR Male mice, 6-8 wk old, were divided at random into 3 groups: A) normal control; B) irradiated with 1 Gy; and C) after 1 Gy irradiation, given CVP 60 mg.kg-1 (ig) daily for 10 d continuously. Body weight (BW), spleen weight (SW), relative SW (RSW), DNA synthesis of splenocytes (DNA-SS), and relative DNA-SS were measured on d 5, 12, 19, 26, and 33 after irradiation. RESULTS: SW, RSW, DNA-SS, and relative DNA-SS decreased after irradiation. CVP enhanced the recovery of SW, RSW, DNA-SS, and relative DNA-SS inhibited by irradiation. CONCLUSION: CVP has the restorative effect against spleen injury induced by gamma-ray irradiation in mice.