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Cancer Biother 1994;9(2):171-178
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Suppressive effects on cancer cell
proliferation of the enhancement of superoxide dismutase (SOD)
activity associated with the protein-bound polysaccharide of
Coriolus versicolor QUEL.
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Kobayashi Y, Kariya K, Saigenji K, Nakamura K
Molecular Biology Laboratory, Kitasato
University School of Medicine, Kanagawa, Japan.
| The protein-bound polysaccharide of Coriolus versicolor QUEL
(PS-K) expresses superoxide dismutase (SOD) mimicking activity.
Examination was made of the suppressive effects of PS-K on cancer
cell lines cultured in vitro. SOD activity of incorporated PS-K
was 5.88 u/mg in LLC-WRC-256 (Walker 256 fibrosarcoma)
cells and 4.73 u/mg in NRK-49F (rat normal kidney fibroblast)
cells. SOD activity in both cell types was enhanced about 7-8
times that of the original PS-K. PS-K was not incorporated into
H4-1 1-E or H4-1 1-E-C3 (rat hepatoma) cells. SOD activity of 1
mg/mi PS-K incubated with cell homogenates of LLC-WRC-256 cells
for 6 hours increased from 0.68 u/mg to 1.35 u/mg. SOD activity
of PS-K 1 mg/mi in 0.05 M phosphate buffer incubated with
50 microM NADPH increased from 0.68 u/mg. The consumption of
NADPH at the same concentration was confirmed
spectrophotometically by incubation with PS-K. The mechanism for
the enhancement of SOD activity associated with PS-K is
considered to be collaboration with NADPH as an electron donor in
the cytoplasm of cancer cells whose SOD and coupling enzyme
activities are significantly lower than in normal cells. |
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